GLYCEROL KINASE AND DIHYDROXYACETONE KINASE IN RAT BRAIN

Glycerol kinase and dihydroxyacetone kinase in rat brain.

The enzymatic phosphorylation of glycerol and dihydroxyacetone by ATP to ,sn-glycerol-3phosphate and dihydroxyacetone phosphate respectively in various subcellular fractions of rat hrain was studied. A sensitive radiochemical assay was used where the labelled phosphorylated products were separated from the radioactive substrates by high voltage paper electrophoresis and the radioactivity in the...

Novel listerial glycerol dehydrogenase- and phosphoenolpyruvate-dependent dihydroxyacetone kinase system connected to the pentose phosphate pathway.

Several bacteria use glycerol dehydrogenase to transform glycerol into dihydroxyacetone (Dha). Dha is subsequently converted into Dha phosphate (Dha-P) by an ATP- or phosphoenolpyruvate (PEP)-dependent Dha kinase. Listeria innocua possesses two potential PEP-dependent Dha kinases. One is encoded by 3 of the 11 genes forming the glycerol (gol) operon. This operon also contains golD (lin0362), wh...

Glycerol kinase activities in rat heart and adipose tissue.

Glycerol Kinase: Substrate Specificityl

Glycerol kinase (8.C. 2.7.1.30, ATP: glycerol-3-phosphotransferase) catalyzes the phosphorylation of 28 nitrogen, sulfur, and alkyl-substituted analogues of glycerol. A survey of 66 analogues has defined qualitatively the structural characteristics nffissary for acceptability as a substrate by this enzyme. This survey was conducted using an assay based on 3lP NMR spectroscopy which detected pro...

The diphosphoinositide kinase of rat brain.

1. The supernatant fraction of adult rat brain contains a diphosphoinositide kinase. 2. Formation of triphosphoinositide by the enzyme in the presence of ATP and Mg(2+) ions was shown with labelled ATP or labelled diphosphoinositide. 3. The kinase was also activated by Ca(2+), Mn(2+) and Co(2+) ions, but to a smaller extent than by Mg(2+) ions. 4. In the presence of optimum Mg(2+) ion concentra...